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one-phase association function in prism  (GraphPad Software Inc)


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    GraphPad Software Inc one-phase association function in prism
    One Phase Association Function In Prism, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/one-phase association function in prism/product/GraphPad Software Inc
    Average 90 stars, based on 1 article reviews
    one-phase association function in prism - by Bioz Stars, 2026-05
    90/100 stars

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    In vitro folded Y 2 R variants functionally activate purified G i protein. (A) Y 2 R folded into isotropic bicelles and activated with NPY drastically accelerates nucleotide exchange of wild type Gα i1 . The fluorescence trace is given as mean of seven independent experiments. (B) Resulting apparent rates of GTPγS binding of Gα i1 (basal) and Y 2 R-catalyzed nucleotide exchange. Statistical significance was determined using one-way ANOVA/Dunnett's post hoc test against basal Gα i1 in Graph Pad Prism 5.03. ### p < 0.001; ## p < 0.01.

    Journal: Frontiers in Molecular Biosciences

    Article Title: Improved in Vitro Folding of the Y 2 G Protein-Coupled Receptor into Bicelles

    doi: 10.3389/fmolb.2017.00100

    Figure Lengend Snippet: In vitro folded Y 2 R variants functionally activate purified G i protein. (A) Y 2 R folded into isotropic bicelles and activated with NPY drastically accelerates nucleotide exchange of wild type Gα i1 . The fluorescence trace is given as mean of seven independent experiments. (B) Resulting apparent rates of GTPγS binding of Gα i1 (basal) and Y 2 R-catalyzed nucleotide exchange. Statistical significance was determined using one-way ANOVA/Dunnett's post hoc test against basal Gα i1 in Graph Pad Prism 5.03. ### p < 0.001; ## p < 0.01.

    Article Snippet: GTPγS binding kinetics was fitted applying the built-in one-phase association function of GraphPad Prism 5.03 (GraphPad Software, San Diego, CA, USA) to obtain the apparent rate constant k.

    Techniques: In Vitro, Purification, Fluorescence, Binding Assay